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Cryo-EM structure determination of small therapeutic protein targets at 3 Å-resolution using a rigid imaging scaffold
Cryoelectron microscopy (Cryo-EM) has enabled structural determination of proteins larger than about 50 kDa, including many intractable …
Roger Castells-Graells, Kyle Meador, Mark A. Arbing, Michael R. Sawaya, Morgan Gee, Duilio Cascio, Emma Gleave, Judit É. Debreczeni, Jason Breed, Karoline Leopold, Ankoor Patel, Dushyant Jahagirdar, Bronwyn Lyons, Sriram Subramaniam, Chris Phillips, Todd O. Yeates
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Cryo-EM structure determination of small therapeutic protein targets at 3 Å-resolution using a rigid imaging scaffold
Design of Beta-2 Microglobulin Adsorbent Protein Nanoparticles
Beta-2 microglobulin (B2M) is an immune system protein that is found on the surface of all nucleated human cells. B2M is naturally shed …
Justin E. Miller, Roger Castells-Graells, Mark A. Arbing, Aldo Munoz, Yi Xiao Jiang, Charlize T. Espinoza, Brian Nguyen, Paul Moroz, Todd O. Yeates
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Design of Beta-2 Microglobulin Adsorbent Protein Nanoparticles
Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus
Many virus capsids undergo exquisitely choreographed maturation processes in their host cells to produce infectious virions, and these …
Roger Castells-Graells, Jonas R.S. Ribeiro, Tatiana Domitrovic, Emma L. Hesketh, Charlotte A. Scarff, John E. Johnson, Neil A. Ranson, David M. Lawson, George P. Lomonossoff
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Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus
A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants
The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies …
Eva C.Thuenemann, Matthew J. Byrne, Hadrien Peyret, Keith Saunders, Roger Castells-Graells, Inmaculada Ferriol, Mattia Santoni, John F.C. Steele, Neil A. Ranson, Linda Avesani, Juan Jose Lopez-Moya, George P. Lomonossoff
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A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants
Plant-based production can result in covalent cross-linking of proteins
Antibodies, antigens and enzymes for replacement therapies and virus-like particles (VLPs) have all been produced successfully in …
Roger Castells-Graells, George P. Lomonossoff
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Plant-based production can result in covalent cross-linking of proteins
Introduction of glucan synthase into the cytosol in wheat endosperm causes massive maltose accumulation and represses starch synthesis
We expressed a bacterial glucan synthase (Agrobacterium GlgA) in the cytosol of developing endosperm cells in wheat grains, to discover …
Tina B. Schreier, Brendan Fahy, Laure C. David, Hamad Siddiqui, Roger Castells-Graells, Alison M. Smith
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Introduction of glucan synthase into the cytosol in wheat endosperm causes massive maltose accumulation and represses starch synthesis
Dynamics and stability in the maturation of a eukaryotic virus: a paradigm for chemically programmed large-scale macromolecular reorganization
Virus maturation is found in all animal viruses and dsDNA bacteriophages that have been studied. It is a programmed process, cued by …
John E. Johnson, Tatiana Domitrovic, Tsutomu Matsui, Roger Castells-Graells, George P. Lomonossoff
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Dynamics and stability in the maturation of a eukaryotic virus: a paradigm for chemically programmed large-scale macromolecular reorganization
Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process
Pathogens modulate plant cell structure and function by secreting effectors into host tissues. Effectors typically function by …
Benjamin Petre, Mauricio P. Contreras, Tolga O. Bozkurt, Martin H. Schattat, Jan Sklenar, Sebastian Schornack, Ahmed Abd-El-Haliem, Roger Castells-Graells, Rosa Lozano-Durán, Yasin F. Dagdas, Frank L.H. Menke, Alexandra M.E. Jones, Jack H. Vossen, Silke Robatzek, Sophien Kamoun, Joe Win
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Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process
OnfD, an AraC-Type Transcriptional Regulator Encoded by Rhizobium tropici CIAT 899 and Involved in Nod Factor Synthesis and Symbiosis
The synthesis and export of rhizobial NF are mediated by a conserved group of LysR-type regulators, the NodD proteins. Here, we have …
Pablo del Cerro, Paula Ayala-García, Pablo Buzón, Roger Castells-Graells, Francisco Javier López-Baena, Francisco Javier Ollero, Francisco Pérez-Montaño
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OnfD, an AraC-Type Transcriptional Regulator Encoded by Rhizobium tropici CIAT 899 and Involved in Nod Factor Synthesis and Symbiosis
High stability of plant-expressed virus-like particles of an insect virus in artificial gastric and intestinal fluids
The harsh conditions of the gastro-intestinal (GI) milieu pose a major barrier to the oral delivery of protein nanocages. Here we …
Alberto Berardi, Roger Castells-Graells, George P. Lomonossoff
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High stability of plant-expressed virus-like particles of an insect virus in artificial gastric and intestinal fluids