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Rigidified Scaffolds for 3 Angstrom Resolution Cryo-EM of Small Therapeutic Protein Targets
We use molecular engineering techniques to rigidify an imaging scaffold, based on a designed protein cage, to the point where 3 A resolution can be achieved with cryo-EM, even for very small proteins.
Roger Castells-Graells, Kyle Meador, Mark A. Arbing, Michael R. Sawaya, Morgan Gee, Duilio Cascio, Emma Gleave, Judit É. Debreczeni, Jason Breed, Chris Phillips, Todd O. Yeates
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Rigidified Scaffolds for 3 Angstrom Resolution Cryo-EM of Small Therapeutic Protein Targets
A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants
The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies …
Eva C.Thuenemann, Matthew J. Byrne, Hadrien Peyret, Keith Saunders, Roger Castells-Graells, Inmaculada Ferriol, Mattia Santoni, John F.C. Steele, Neil A. Ranson, Linda Avesani, Juan Jose Lopez-Moya, George P. Lomonossoff
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A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants
Introduction of glucan synthase into the cytosol in wheat endosperm causes massive maltose accumulation and represses starch synthesis
We expressed a bacterial glucan synthase (Agrobacterium GlgA) in the cytosol of developing endosperm cells in wheat grains, to discover …
Tina B. Schreier, Brendan Fahy, Laure C. David, Hamad Siddiqui, Roger Castells-Graells, Alison M. Smith
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Introduction of glucan synthase into the cytosol in wheat endosperm causes massive maltose accumulation and represses starch synthesis
Dynamics and stability in the maturation of a eukaryotic virus: a paradigm for chemically programmed large-scale macromolecular reorganization
Virus maturation is found in all animal viruses and dsDNA bacteriophages that have been studied. It is a programmed process, cued by …
John E. Johnson, Tatiana Domitrovic, Tsutomu Matsui, Roger Castells-Graells, George P. Lomonossoff
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Dynamics and stability in the maturation of a eukaryotic virus: a paradigm for chemically programmed large-scale macromolecular reorganization
Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process
Pathogens modulate plant cell structure and function by secreting effectors into host tissues. Effectors typically function by …
Benjamin Petre, Mauricio P. Contreras, Tolga O. Bozkurt, Martin H. Schattat, Jan Sklenar, Sebastian Schornack, Ahmed Abd-El-Haliem, Roger Castells-Graells, Rosa Lozano-Durán, Yasin F. Dagdas, Frank L.H. Menke, Alexandra M.E. Jones, Jack H. Vossen, Silke Robatzek, Sophien Kamoun, Joe Win
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Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process
OnfD, an AraC-Type Transcriptional Regulator Encoded by Rhizobium tropici CIAT 899 and Involved in Nod Factor Synthesis and Symbiosis
The synthesis and export of rhizobial NF are mediated by a conserved group of LysR-type regulators, the NodD proteins. Here, we have …
Pablo del Cerro, Paula Ayala-García, Pablo Buzón, Roger Castells-Graells, Francisco Javier López-Baena, Francisco Javier Ollero, Francisco Pérez-Montaño
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OnfD, an AraC-Type Transcriptional Regulator Encoded by Rhizobium tropici CIAT 899 and Involved in Nod Factor Synthesis and Symbiosis
NRC4 Gene Cluster Is Not Essential for Bacterial Flagellin-Triggered Immunity
Plants utilize cell surface pattern recognition receptors (PRRs) and intracellular nucleotide-binding domain Leu-rich repeat containing …
Chih-Hang Wu, Hiroaki Adachi, Juan Carlos De la Concepcion, Roger Castells-Graells, Vladimir Nekrasov, Sophien Kamoun
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NRC4 Gene Cluster Is Not Essential for Bacterial Flagellin-Triggered Immunity
Production of Mosaic Turnip Crinkle Virus-Like Particles Derived by Coinfiltration of Wild-Type and Modified Forms of Virus Coat Protein in Plants
When the coat protein reading frame of turnip crinkle virus (TCV) is transiently expressed in leaves, virus-like particles (VLPs) are …
Roger Castells-Graells, George P. Lomonossoff, Keith Saunders
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Production of Mosaic Turnip Crinkle Virus-Like Particles Derived by Coinfiltration of Wild-Type and Modified Forms of Virus Coat Protein in Plants
Synthetic plant virology for nanobiotechnology and nanomedicine
Nanotechnology is a rapidly expanding field seeking to utilize nano-scale structures for a wide range of applications. Biologically …
John F.C Steele,, Hadrie Peyret, Keith Saunders, Roger Castells-Graells, Johanna Marsian, Yulia Meshcheriakova, George P. Lomonossoff
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Synthetic plant virology for nanobiotechnology and nanomedicine
The Impact of Transposable Elements in the Evolution of Plant Genomes: From Selfish Elements to Key Players
Transposable elements (TEs) are major components of all eukaryote genomes, and in particular of plant genomes. Whereas these elements …
Beatriz Contreras, Cristina Vives, Roger Castells-Graells, Josep M. Casacuberta
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The Impact of Transposable Elements in the Evolution of Plant Genomes: From Selfish Elements to Key Players